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Effect of MDM2 overexpression on response to etoposide treatment

GSE308508 Mus musculus Expression profiling by high throughput sequencing 8 samples Submitted 2025/09/23 Platform GPL34290
Summary
We aimed to study the effect of non-mutational p53 inactivation on the response of hematopoietic progenitor cells to genotoxic treatment. In order to study this in vitro, we generated HSPC lines by enforced ER-Hoxb8 expression in bone marrow cells derived from transgenic Trp53-fl-R245W-GFP mice. In the absence of Cre-mediated recombination, these cells are wild-type for Trp53. We additionally transduced these cells lentivirally with a construct overexpressing murine Mdm2 or a control vector. This leads to non-mutational inactivation of functional p53. Thereby, we aimed to compare the transcriptional response to genotoxic treatment in Trp53 wild-type cells with or without non-mutational p53 inactivation through Mdm2 overexpression
Published in
The pathogenesis of therapy-related myeloid neoplasms from TP53-mutant clonal hematopoiesis
Fullin J, Topçu E, Zielińska KA et al. · Leukemia 2026 · PMID 41407825 · doi:10.1038/s41375-025-02839-5
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Also filed as BioProject PRJNA1330791 and SRA study SRP621908. Searching any of these in the dataset finder brings you back here.

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