Bulk RNA-seq of WNT-responsive and WNT-secreting cancer cell populations from orthotopic PDAC tumors
RNA-seq (gene expression) · mouse · 16 samples
- What it is
- RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
- Organism
- Mus musculus (mouse)
- Samples
- 16 samples: 16 samples, each with a distinct title — no replicated groupsGroups are read from the sample names, not from the paper. Check the list below.
- Data you can get
- A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
- Paper
- Disruption of WNT/Notch signaling in pancreatic cancer reveals tumors depend on the intricate equilibrium of malignant cell statesDevelopmental cell · 2026
Read the full summary ↓
Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.
Processed table
The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.
Supplementary files
The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE316194_RAW.tar (it can run to tens of gigabytes).
Raw sequencing reads
The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.
What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.
Also filed as BioProject PRJNA1401785 and SRA study SRP661493. Searching any of these in the dataset finder brings you back here.
Any grouping that defines a comparison lives in the sample characteristics, which GEO records separately from the titles.
How we read this: Read from 16 sample titles: 16 distinct titles with little repetition. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.
- GSM9447212 Sorted_1_Dneg
- GSM9447213 Sorted_1_Dpos
- GSM9447214 Sorted_1_GFP
- GSM9447215 Sorted_1_mScarlet
- GSM9447216 Sorted_2_Dneg
- GSM9447217 Sorted_2_Dpos
- GSM9447218 Sorted_2_GFP
- GSM9447219 Sorted_2_mScarlet
- GSM9447220 Sorted_3_Dneg
- GSM9447221 Sorted_3_Dpos
- GSM9447222 Sorted_3_GFP
- GSM9447223 Sorted_3_mScarlet
- GSM9447224 Sorted_4_Dneg
- GSM9447225 Sorted_4_Dpos
- GSM9447226 Sorted_4_GFP
- GSM9447227 Sorted_4_mScarlet
- GSE232196 Differential expression gene signature between primary tumors and metastatic tumors in PDAC and BC mouse models [Bulk Tissue RNA-Seq] 20 samples shares pdac, rna-seq, bulk
- GSE262879 Single-cell RNA-sequencing of murine pancreatic tumors derived from the orthotopic transplantation of Smad4 wild-type (WT) or Smad4 knockout (KO) pancreatic ductal adenocarcinoma (PDAC) KPC organoids in nu/nu mice. 18 samples shares pdac, orthotopic, rna-seq
- GSE329640 Bulk RNA-sequencing analyses of multi-stroma flow-sorted from cocultures with murine pancreatitis (P)-derived organoids, PDAC organoids, or PDAC organoids following initial coculture with P organoids (ie P-to-PDAC or sequential). 12 samples shares pdac, rna-seq, bulk
- GSE222222 Bulk RNA-seq analysis of IFNγ-PD-L1all, IFNγ+PD-L1all and IFNγ+PD-L1hi PDAC cells 10 samples shares pdac, rna-seq, bulk
- GSE262878 Single-cell RNA-sequencing of murine pancreatic tumors derived from the orthotopic transplantation of Smad4 wild-type (WT) or Smad4 knockout (KO) pancreatic ductal adenocarcinoma (PDAC) KvPC organoids in nu/nu mice 8 samples shares pdac, orthotopic, rna-seq
- GSE329665 Single-nuclei RNA-sequencing (snRNA-seq) of murine pancreatic tumors derived from the orthotopic transplantation of Smad4 WT or Smad4 KO pancreatic ductal adenocarcinoma (PDAC) KPC (i.e. KrasG12D; p53 mutant) organoids in C57BL/6J mice. 6 samples shares pdac, orthotopic, rna-seq
- GSE205410 Multiple subpopulations in medullary thymic epithelial cells are regulated by Aire with distinctive modes of gene regulations [bulkRNA-seq] 47 samples shares populations, rna-seq, bulk
- GSE228317 Medullary cavity expansion is mediated by distinct cell populations during fetal bone development [bulk RNA-seq] 20 samples shares populations, rna-seq, bulk
Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.