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Single‑cell RNA sequencing of orthotopic GBM tumors following oncolytic adenovirus treatment

GSE316315 Mus musculus Expression profiling by high throughput sequencing 9 samples Submitted 2026/01/20 Platform GPL34290
Summary
Mice bearing intracranial GL261‑CAR tumors were treated intratumorally with PBS (control), oAd, or oAd‑mCD47/PD‑L1‑mIL‑12 (1 × 109 pfu). Tumors were harvested three days after the second viral injection and dissociated into single‑cell suspensions using the SeekMate Tissue Dissociation Reagent Kit A Pro (SeekGene, K01801‑30).Single‑cell RNA‑seq libraries were constructed using the SeekOne Digital Droplet Single‑Cell 3′ Library Preparation Kit (SeekGene, #K00202).Single‑cell transcriptomic data demonstrate that oAd‑mCD47/PDL1‑mIL‑12 orchestrates a multi‑faceted immune response: expanding cytotoxic and memory T‑cell subsets, polarizing macrophages toward an antigen‑presenting and chemokine‑secreting phenotype, and enhancing reciprocal signaling between immune and cancer cells to promote tumor clearance.
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Direct links to NCBI, no account and no request form: the whole study as GSE316315_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 9 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA1402281 and SRA study SRP661839. Searching any of these in the dataset finder brings you back here.

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