Transcriptomic analysis of hippocampus and prefrontal cortex in a mouse model of chronic radiation-induced gastrointestinal injury

Chronic radiation-induced gastrointestinal injury is associated with persistent gut dysfunction and microbiome alterations, which may contribute to extra-intestinal complications including neurovascular and behavioral abnormalities. To investigate molecular changes in the brain during chronic radiation-induced intestinal injury, RNA sequencing was performed using hippocampal and prefrontal cortex tissues collected from irradiated mice and non-irradiated controls. Transcriptomic profiling revealed region-specific alterations, with the hippocampus showing more pronounced changes in pathways related to vascular remodeling, extracellular matrix organization, inflammatory response, and blood–brain barrier dysfunction. These datasets provide a resource for understanding gut–brain axis-associated molecular alterations in chronic radiation-induced injury.