GEO series

PACAP-PAC1R signaling in the PSTh mediates stress-induced anxiety-like behavior in mice

GSE332821 Mus musculus Expression profiling by high throughput sequencing 4 samples Submitted 2026/07/20 Platform GPL24247

Summary

Stress represents a major risk factor for anxiety disorders, yet the underlying molecular substrates through which stress causes anxiety remain elusive. We employed an established stress paradigm to induce anxiety-like behaviors in mice and found that stress causes robust and sustained activation of glutamatergic neurons in the parasubthalamic nucleus (PSTh). Moreover, inhibition of those neurons significantly reduced anxiety-like behavior in stressed animals. RNA sequencing revealed that the pituitary adenylate cyclase-activating polypeptide type 1 receptor (PAC1R, encoded by Adcyap1r1) is enriched in the PSTh. Ex vivo patch-clamp recordings showed that PACAP, an endogenous agonist of PAC1R, increases the excitability of PSTh neurons. Interestingly, pharmacological blockade of PAC1R within PSTh was sufficient to prevent anxiety by stress. These findings suggest that stress induces sustained activation of PSTh neurons PACAP-PAC1R signaling, ultimately leading to anxiety-like state.

This dataset

Open GSE332821 in finder → Find more mouse RNA-seq datasets →

Download

Direct links to NCBI, no account and no request form: the whole study as GSE332821_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 4 samples. Raw sequencing reads are also available from ENA.

Whole study · RAW.tar ↓ Series matrix ↓ All supplementary files ↗ Raw reads on ENA ↗

Also filed as BioProject PRJNA1469131 and SRA study SRP702704. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗

Samples in this study

The sample list for this study is not cached yet. Press Sort into groups and it will be fetched from NCBI.

+ 4 more — browse all 4 samples with per-sample file links →

Similar datasets

Search all mouse RNA-seq datasets in GEO →

Share this dataset

Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.