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Systematic Identification of Defined Conditions for Induction and Maintenance of Naive Human Pluripotency [expression]

GSE59430 Homo sapiens Expression profiling by array 7 samples Submitted 2014/07/16 Platform GPL16043
Summary
Embryonic stem cells (ESCs) of mice and humans have distinct molecular and biological characteristics, raising the question whether an earlier ‘naive’ state of pluripotency may exist in humans. Here we took a systematic approach to identify small molecules that support autonomous self-renewal of naive human ESCs based on maintenance of endogenous OCT4 distal enhancer activity, a molecular signature of ground state pluripotency. Iterative chemical screening identified a combination of five kinase inhibitors that induces and maintains OCT4 distal enhancer activity when applied directly to conventional human ESCs. These inhibitors generate a homogeneous population of human pluripotent stem cells in which transcription factors associated with the ground state of pluripotency are highly upregulated. Comparison with previously reported naive human ESCs indicates that our kinase inhibitor cocktail captures a novel pluripotent state in humans that closely resembles mouse ESCs.
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Also filed as BioProject PRJNA255307. Searching any of these in the dataset finder brings you back here.

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