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The Transcriptome and DNA Methylome Landscapes of Human Primordial Germ Cells

GSE63818 Homo sapiens Expression profiling by high throughput sequencing; Methylation profiling by high throughput sequencing 364 samples Submitted 2015/06/03 Platform GPL16791Platform GPL11154
Summary
Germ cells are vital for transmitting genetic information from one generation to the next and for maintaining the continuation of species. Here, we analyze the transcriptome of human primordial germ cells (PGCs) from the migrating stage to the gonadal stage at single-cell and single-base resolutions. Human PGCs show unique transcription patterns involving the simultaneous expression of both pluripotency genes and germline-specific genes, with a subset of them displaying developmental stage-specific features. Furthermore, we analyze the DNA methylome of human PGCs and find global demethylation of their genomes. Approximately 10-11 weeks after gestation, the PGCs are nearly devoid of any DNA methylation; with only 7.8% and 6.0% of the median methylation levels in male and female PGCs, respectively. Our work paves the way toward deciphering the complex epigenetic reprogramming of the germline with the aim of restoring totipotency in fertilized oocytes.
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Direct links to NCBI, no account and no request form: the whole study as GSE63818_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 364 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA269154 and SRA study SRP050499. Searching any of these in the dataset finder brings you back here.

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