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Single-Cell RNA-Seq Analysis Maps Development of Human Germline Cells and Gonadal- Niche Interactions

GSE86146 Homo sapiens Expression profiling by high throughput sequencing 43 samples Submitted 2017/03/31 Platform GPL16791
Summary
Human fetal germ cells (FGCs) are precursors to sperm and eggs and are crucial for maintenance of the species. However, the developmental trajectories and heterogeneity of human FGCs remain largely unknown. Here, we performed single-cell RNA-seq analysis of over 2000 FGCs and their gonadal niche cells in female and male human embryos spanning several developmental stages. We found that the female FGCs undergo four distinct sequential phases characterized by mitosis, retinoic acid signaling, meiotic prophase, and oogenesis. Male FGCs develop through stages of migration, mitosis and cell cycle arrest. Individual embryos of both sexes simultaneously contain several subpopulations, highlighting the asynchronous and heterogeneous nature of FGC development. Moreover, we observed reciprocal signaling interactions between FGCs and their gonadal niche cells, including activation of BMP and Notch signaling pathways. Our work provides key insights into crucial features of human FGCs during their highly ordered mitotic, meiotic, and gametogenetic processes in vivo.
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Direct links to NCBI, no account and no request form: the whole study as GSE86146_RAW.tar, processed values as the series matrix, the supplementary file directory, and per-sample supplementary files for any of the 43 samples. Raw sequencing reads are also available from ENA.

Also filed as BioProject PRJNA340388 and SRA study SRP083134. Searching any of these in the dataset finder brings you back here.

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