In vitro neuronal RNA-sequencing, in vivo cortical single nucleus RNA-sequencing, in vivo cortical ChIP-sequencing of H3K79me2 in models of DOT1L loss
ChIP-seq / ATAC-seq (genome binding and open chromatin) · mouse · 26 samples
- What it is
- ChIP-seq / ATAC-seq (genome binding and open chromatin)Where proteins bind the genome, or which regions are open and active.
- Organism
- Mus musculus (mouse)
- Samples
- 26 samples: 10 conditions, mostly in duplicateGroups are read from the sample names, not from the paper. Check the list below.
- Data you can get
- A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
- Paper
- Loss of DOT1L disrupts neuronal transcription and leads to a neurodevelopmental disorderBrain : a journal of neurology · 2026
Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.
Processed table
The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.
Supplementary files
The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE279978_RAW.tar (it can run to tens of gigabytes).
Raw sequencing reads
The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.
What the files usually look like: Look for peak files (.bed, .narrowPeak) and signal tracks (.bigWig) among the supplementary files; the raw reads are on ENA.
Also filed as BioProject PRJNA1175649 and SRA study SRP572229. Searching any of these in the dataset finder brings you back here.
Supports a between-group comparison across 22 samples.
How we read this: 10 replicated groups read from 26 sample titles; they account for 22 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.
- GSM8414020 Ctrl Luciferase bio rep 1 Ctrl Luciferase
- GSM8414021 Ctrl Luciferase bio rep 2 Ctrl Luciferase
- GSM8414022 Ctrl Luciferase bio rep 3 Ctrl Luciferase
- GSM8414023 DOT1L shRNA bio rep 1 DOT1L shRNA
- GSM8414024 DOT1L shRNA bio rep 2 DOT1L shRNA
- GSM8414025 DOT1L shRNA bio rep 3 DOT1L shRNA
- GSM8561774 Control
- GSM8561775 Dot1l HET
- GSM8862381 Control_Female_H3K79me2_R1 Control_Female_H3K79me2
- GSM8862382 HET_Female_H3K79me2_R1 HET_Female_H3K79me2
- GSM8862383 Control_Female_H3K79me2_R2 Control_Female_H3K79me2
- GSM8862384 HET_Female_H3K79me2_R2 HET_Female_H3K79me2
- GSM8862385 Control_Male_H3K79me2_R3
- GSM8862386 HET_Male_H3K79me2_R3 HET_Male_H3K79me2
- GSM8862387 Control_Male)H3K79me2_R4
- GSM8862388 HET_Male_H3K79me2_R4 HET_Male_H3K79me2
- GSM8862389 Control_Female_input_R1 Control_Female_input
- GSM8862390 HET_Female_input_R1 HET_Female_input
- GSM8862391 Control_Female_input_R2 Control_Female_input
- GSM8862392 HET_Female_input_R2 HET_Female_input
- GSM8862393 Control_Male_input_R3 Control_Male_input
- GSM8862394 HET_Male_input_R3 HET_Male_input
- GSM8862395 Control_Male_input_R4 Control_Male_input
- GSM8862396 HET_Male_input_R4 HET_Male_input
- GSM8862397 IgG_control_R1 IgG_control
- GSM8862398 IgG_control_R2 IgG_control
- GSE306634 DOT1L-mediated H3K79me2 directs B-cell repertoire establishment, marginal zone development and germinal center function 21 samples shares h3k79me2, dot1l
- GSE301374 Inhibition of H3K79me2 by DOT1L inhibitor EPZ5676 promotes mouse embryonic lung branching morphogenesis via increasing epithelium proliferation 9 samples shares h3k79me2, dot1l
- GSE292609 ChIP-seq analysis of control and DOT1L HET cortical nuclei. 18 samples shares dot1l, cortical
- GSE279120 single-nucleus transcriptomic analysis of cortical tissue from control and Dot1l HET 10 week old mice. 2 samples shares dot1l, cortical
- GSE213012 Total and dendritic RNA-sequencing from primary mouse cortical neurons 32 samples shares rna-sequencing, cortical
- GSE224041 ST105-01: UNBIASED IN-VIVO AND IN-SILICO TL-532 MECANISM OF ACTION BY RNA-SEQUENCING 12 samples shares rna-sequencing, vivo
- GSE222235 DOT1L controls NK cell maturation and lineage integrity 27 samples shares dot1l
- GSE233975 DOT1L activity limits transcription elongation velocity and favors RNAPII pausing to facilitate mutagenesis by AID 45 samples shares dot1l
Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.