← BioTransfer GEO Dataset Finder
GEO series

Investigating Trophoblast Invasion and Angiogenesis Expression Changes in a Caloric Deficient Mouse Model of Fetal Growth Restriction

GSE315973 Mus musculus (mouse) RNA-seq (gene expression) 10 samples Submitted 2026/01/08 Platform GPL24247
At a glance

RNA-seq (gene expression) · mouse · 10 samples

What it is
RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism
Mus musculus (mouse)
Samples
10 samples
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper
Investigating trophoblast invasion and angiogenesis expression changes in a caloric deficient mouse model of fetal growth restrictionReproductive biology · 2026
Summary (written by the submitters)
Fetal growth restriction (FGR) is a severe pregnancy complication often caused by placental insufficiency. Proper trophoblast invasion is essential for placental development and function, ensuring adequate nutrient and oxygen supply to the developing fetus. Dysregulation impairs placental perfusion, leading to FGR. This study uses a calorie-restricted mouse model to investigate genes/molecular mechanisms regulating trophoblast invasion across gestational timepoints.
Read the full summary ↓
Pregnant mice received either a standard or 50% calorie-restricted diet from E8.5. Placentas and invasion sites were analyzed at E10.5, E12.5, E14.5, E16.5, and E17.5. mRNA sequencing and RT/qPCR examined trophoblast invasion-related genes (Mmp2, Mmp9, Efna1, Rac1, Rras, Ascl2, Tfap2c, Prl7b1) and angiogenesis genes (Vegfa, Vegfb, Pdgf, Akt3). Immunohistochemistry of trophoblast cells (cytokeratin 8, CK8) and endothelial cell markers (endomucin, CD31, CCD105, VEGFR2) was performed. Statistical analysis used Student’s t-test. Caloric restriction significantly reduced fetal/placental weights from E12.5, with persistent growth restriction at E16.5, and E17.5. IHC at E17.5 showed reduced decidual depth, trophoblast invasion distance, and trophoblast quantity within the decidua. This impaired growth was accompanied by reduced expression of trophoblast invasion genes (Mmp2, Mmp9, Efna1, Rac1, Rras, Ascl2, Tfap2c, Prl7b1) in FGR placentas, with a reduction in CK8 trophoblast staining. Angiogenesis reduction in FGR was demonstrated with reduced Vegfa, Vegfb, and Akt3 and supported by reduced CD31, CD105, and VEGF2 endothelial cell markers A caloric-restriction mouse model replicates key FGR pathophysiology, including reduced fetal/placental growth, downregulation of trophoblast invasion genes, impaired trophoblast invasion into the decidua, and reduced placenta angiogenesis. These findings offer molecular insights into placental insufficiency that merits further exploration regarding FGR pathogenesis.
Published in
Investigating trophoblast invasion and angiogenesis expression changes in a caloric deficient mouse model of fetal growth restriction
Bardill JR, Eason CR, Wood H et al. · Reproductive biology 2026 · PMID 41576455 · doi:10.1016/j.repbio.2026.101179
This dataset
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE315973_RAW.tar (it can run to tens of gigabytes).

3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1400045 and SRA study SRP660735. Searching any of these in the dataset finder brings you back here.

Samples in this study

The sample list for this study is not cached yet. Press Load samples and it will be fetched from NCBI.

+ 10 more — browse all 10 samples with per-sample file links →

Datasets on a similar topic

Search all mouse RNA-seq datasets in GEO →

Share this dataset

Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.