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ChREBP Is Dispensable for Myofiber Type-switch but Promotes Skeletal Muscle Regeneration

GSE319093 Mus musculus (mouse) RNA-seq (gene expression) 62 samples Submitted 2026/07/01 Platform GPL9185
At a glance

RNA-seq (gene expression) · mouse · 62 samples

What it is
RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism
Mus musculus (mouse)
Samples
62 samples: 62 samples, each with a distinct title — no replicated groupsGroups are read from the sample names, not from the paper. Check the list below.
Data you can get
A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper
ChREBP Is Dispensable for Myofiber Type Switch but Promotes Skeletal Muscle RegenerationNutrients · 2026
Summary (written by the submitters)
Muscle performance and fatigability are determined by the structural and metabolic properties of the specialized myofibers. Skeletal muscle demonstrates significant plasticity, with myofiber types and energy metabolic functions undergoing adaptive remodeling in response to physiological or pathophysiological stimuli. The transcription factor carbohydrate-responsive element-binding protein (ChREBP) is recognized as a crucial glucose-sensing regulator involved in glucose and lipid metabolism.
Read the full summary ↓
However, However, the precise role of ChREBP in influencing myofiber composition and energy metabolism within muscle tissue remains ambiguous. Here, utilizing skeletal muscle-specific knockout mice, we demonstrated that ChREBP deletion did not substantially alter muscle fiber composition or metabolic function, nor did it play a role in skeletal muscle adaptive remodeling under hypoxic or high-fructose dietary conditions. Interestingly, the absence of ChREBP was associated with a modest enhancement in aerobic exercise capacity, although it markedly impaired lipid transport function and led to a reduction in triglyceride content. Furthermore, we observed that upregulation of ChREBP expression significantly increased the number of newly formed myofibers and their cross-sectional area. Taken together, this study elucidates the role of ChREBP in skeletal muscle and its adaptive remodeling, while also identifying a significant promotive function of ChREBP in the repair of skeletal muscle injuries.
Published in
ChREBP Is Dispensable for Myofiber Type Switch but Promotes Skeletal Muscle Regeneration
Lu J, Chen J, Zhang G et al. · Nutrients 2026 · PMID 42356398 · doi:10.3390/nu18122012
This dataset
Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE319093_RAW.tar (it can run to tens of gigabytes).

3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1422086 and SRA study SRP676250. Searching any of these in the dataset finder brings you back here.

Study design
62 samples, each with a distinct title — no replicated groups

Supports a case/control comparison: 4 samples read as cases, 8 as controls.

How we read this: Read from 62 sample titles: 62 distinct titles with little repetition. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study

+ 22 more — browse all 62 samples with per-sample file links →

Datasets on a similar topic

Search all mouse RNA-seq datasets in GEO →

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Metadata from NCBI GEO, cached and refreshed periodically — the NCBI page above is authoritative. Downloads link straight to NCBI/ENA; nothing is proxied through BioTransfer.