GEO series

Vasculogenic tissue nanotransfection accelerates functional recovery after peripheral nerve injury

GSE319284 Mus musculus (mouse) RNA-seq (gene expression) 18 samples Submitted 2026/03/04 Platform GPL34290

At a glance

RNA-seq (gene expression) · mouse · 18 samples

What it is: RNA-seq (gene expression)Gene activity measured by sequencing. The summary does not mention single-cell work, so this is most likely bulk: one average per sample, the usual starting point for comparing groups.
Organism: Mus musculus (mouse)
Samples: 18 samples: 5 conditions, mostly with about 4 replicates eachGroups are read from the sample names, not from the paper. Check the list below.
Data you can get: A processed table (series matrix) and supplementary files from NCBI; the raw sequencing reads from ENA. Which of these hold counts depends on the study, so check the file list.
Paper: Vasculogenic tissue nanotransfection accelerates functional recovery after peripheral nerve injuryScience advances · 2026

Summary (written by the submitters)

Peripheral nerve injuries (PNI) often result in persistent sensory, motor, and functional deficits, even after surgical reconstruction. While gene and cell therapies offer promise for enhancing regenerative outcomes, effective and targeted delivery remains a significant challenge. Here, we explored vasculogenic Tissue Nano-Transfection (TNT) as a novel, non-viral, reprogramming-based cell therapy to improve outcomes following graft reconstruction surgery.

Read the full summary ↓

TNT aims to improve nerve regeneration by inducing vasculogenic reprogramming and promoting neovascularization to support axonal growth. Through a combination of in vitro screening and in vivo testing in a nerve crush model, we identified an optimal formulation of the vasculogenic genes, Etv2, Fli1, and Foxc2 (EFF), for enhancing reprogramming and axonal repair. TNT-based deployment EFF in a long segmental nerve defect reconstructed with isografts resulted in significant improvements in functional recovery, including grip strength and muscle contractility. Histological analyses demonstrated increased vascular density and myelinated axon counts. These findings support the therapeutic potential of TNT-mediated vasculogenic reprogramming as an adjunct to surgical repair of complex PNI offering a promising strategy to improve nerve regeneration and functional outcomes.

Published in

Vasculogenic tissue nanotransfection accelerates functional recovery after peripheral nerve injury

Salazar-Puerta AI, Kheirkhah S, Stranan JP et al. · Science advances 2026 · PMID 41950328 · doi:10.1126/sciadv.aeb7631

This dataset

Open GSE319284 in finder → Find more mouse RNA-seq datasets →

Download · which file do I need?

Direct links to NCBI and ENA, no account and no request form. Pick by what you want to do: take the small processed table to look, the supplementary files to re-analyse, or the original raw data to start over.

1 · Just have a look

Processed table

The series matrix: one table of values per sample as the submitters processed it. The smallest download, and it opens in Excel or R. Start here to see what the data look like.

Series matrix ↓

2 · Re-analyse it

Supplementary files

The count tables and other files the authors uploaded. Take only the samples you need, or the whole study as GSE319284_RAW.tar (it can run to tens of gigabytes).

All supplementary files ↗ Whole study · RAW.tar ↓

3 · Start from the raw reads

Raw sequencing reads

The original FASTQ files, straight from the sequencer, held at ENA. Large, and you align them yourself, so only choose this if you need to re-process the study from scratch.

Raw reads on ENA ↗

What the files usually look like: For RNA-seq the counts are usually a table among the supplementary files (look for 'counts', 'expression', 'FPKM' or 'TPM' in the file name). The series matrix of a sequencing study is sometimes nearly empty.

Also filed as BioProject PRJNA1422744 and SRA study SRP676557. Searching any of these in the dataset finder brings you back here.

NCBI GEO page ↗ Paper (PMID 41950328) ↗

Study design

5 conditions, mostly with about 4 replicates each

SN_Control ×4 SN_Graft ×4 SN_Sham ×4 SN_EFF ×3 SN_Cut ×3

Supports a between-group comparison across 18 samples.

How we read this: 5 replicated groups read from 18 sample titles; they account for 18 of them. These groups are guessed from the sample names, not taken from the paper, so check them against the sample list below before relying on them.

Samples in this study

GSM9514820 SN_Control_2 SN_Control
GSM9514821 SN_Control_3 SN_Control
GSM9514822 SN_Control_4 SN_Control
GSM9514823 SN_Control_5 SN_Control
GSM9514824 SN_Graft_1 SN_Graft
GSM9514825 SN_Graft_2 SN_Graft
GSM9514826 SN_Graft_4 SN_Graft
GSM9514827 SN_Graft_5 SN_Graft
GSM9514828 SN_Sham_2 SN_Sham
GSM9514829 SN_Sham_3 SN_Sham
GSM9514830 SN_Sham_4 SN_Sham
GSM9514831 SN_Sham_5 SN_Sham
GSM9514832 SN_EFF_1 SN_EFF
GSM9514833 SN_EFF_2 SN_EFF
GSM9514834 SN_EFF_3 SN_EFF
GSM9514835 SN_Cut_1 SN_Cut
GSM9514836 SN_Cut_2 SN_Cut
GSM9514837 SN_Cut_3 SN_Cut

Datasets on a similar topic

GSE317915 Vasculogenic tissue nanotransfection accelerates functional recovery after peripheral nerve injury 16 samples shares nanotransfection, vasculogenic, accelerates, peripheral, recovery, injury, nerve
GSE319328 Vasculogenic tissue nanotransfection accelerates functional recovery after peripheral nerve injury 16 samples shares nanotransfection, vasculogenic, accelerates, peripheral, recovery, injury, nerve
GSE273220 Peripheral nerve injury induces dystonia-like movements and dysregulation in the energy metabolism: A multi-omics descriptive study in Thap1+/− mice 72 samples shares peripheral, injury, nerve
GSE241361 Sigma-1 Receptors Control Neuropathic Pain and Peripheral Neuroinflammation After Nerve Injury in Female Mice: A Transcriptomic Study 36 samples shares peripheral, injury, nerve
GSE312514 Acutely denervated muscle EVs reshape neuronal mitochondrial metabolism via retrograde signaling to rescue peripheral nerve injury 24 samples shares peripheral, injury, nerve
GSE269326 Nucleus Accumbens MEF2C Mediates the Maintenance of Peripheral Nerve Injury-induced Physiological and Behavioral Maladaptations 24 samples shares peripheral, injury, nerve
GSE249114 TFEB/3 govern repair Schwann cell generation and function following peripheral nerve injury 12 samples shares peripheral, injury, nerve
GSE172064 Comparing axon regeneration in male and female mice after peripheral nerve injury 12 samples shares peripheral, injury, nerve

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