RNA-seq profiling of mouse small intestine treated with intravenous immunoglobulin (IVIg) after total abdominal irradiation

Radiation-induced enteritis (RIE) is a common and severe adverse effect of radiotherapy for abdominal and pelvic tumors, with limited effective treatments. We previously demonstrated that intravenous immunoglobulin (IVIg) alleviates radiation toxicity, but its mechanism of action on intestinal stem cell (ISC) regeneration remains unclear. Here, we performed RNA-seq on the small intestine of mice with radiation-induced enteritis to investigate the transcriptomic changes induced by IVIg treatment. Total abdominal irradiation (TAI, 12 Gy) was performed on C57BL/6J mice, followed by intravenous administration of IVIg (500 mg/kg) within 1 h after irradiation. Jejunal tissues were collected on Day 3 post-irradiation for RNA-seq analysis. Our results show that IVIg treatment significantly altered the expression of genes related to cell proliferation, inflammation, oxidative stress, and the Wnt/β-catenin signaling pathway, which is critical for ISC maintenance and regeneration. These data provide a molecular basis for the radioprotective effect of IVIg in mitigating RIE, supporting its potential as a therapeutic candidate for this condition.